Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Journal of Translational Medicine

Article Title: Clinical use of Dieletrophoresis separation for live Adipose derived stem cells

doi: 10.1186/1479-5876-10-99

Figure Lengend Snippet: Schematic drawing of microelectrodes (black rectangles) trapping a stem cell (green sphere) within a DEP field (dotted lines) in the absence of any laminar flow across the array.

Article Snippet: Aliquots of pre- and post-DEP treated cells were analyzed by Guava-PCA flow cytometry using ViaCount(R) (Millipore 4000–0040 LOT 11–0115) reagent or monoclonal anti-human CD34 directly conjugated to phycoerythrin (R&D Systems FAB7227P LOT: ACOG01) according to manufacturer protocol.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Clinical use of Dieletrophoresis separation for live Adipose derived stem cells

doi: 10.1186/1479-5876-10-99

Figure Lengend Snippet: Schematic drawing of `microelectrodes (black rectangles) trapping a stem cell (green sphere) within a DEP field (dotted lines) in the presence of laminar flow (black arrows) across the array. The cell is displaced laterally to the trailing edge of the DEP field.

Article Snippet: Aliquots of pre- and post-DEP treated cells were analyzed by Guava-PCA flow cytometry using ViaCount(R) (Millipore 4000–0040 LOT 11–0115) reagent or monoclonal anti-human CD34 directly conjugated to phycoerythrin (R&D Systems FAB7227P LOT: ACOG01) according to manufacturer protocol.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Clinical use of Dieletrophoresis separation for live Adipose derived stem cells

doi: 10.1186/1479-5876-10-99

Figure Lengend Snippet: Flow cytometry for detection of CD34 cells in DEP isolated SVF (lower panel) versus non-treated SVF (middle panel) and background control (upper panel).

Article Snippet: Aliquots of pre- and post-DEP treated cells were analyzed by Guava-PCA flow cytometry using ViaCount(R) (Millipore 4000–0040 LOT 11–0115) reagent or monoclonal anti-human CD34 directly conjugated to phycoerythrin (R&D Systems FAB7227P LOT: ACOG01) according to manufacturer protocol.

Techniques: Flow Cytometry, Isolation, Control

Journal: Journal of Translational Medicine

Article Title: Clinical use of Dieletrophoresis separation for live Adipose derived stem cells

doi: 10.1186/1479-5876-10-99

Figure Lengend Snippet: CD34 cell counts expressed as a percentage cell population determined by flow cytometry, before and after DEP isolation.

Article Snippet: Aliquots of pre- and post-DEP treated cells were analyzed by Guava-PCA flow cytometry using ViaCount(R) (Millipore 4000–0040 LOT 11–0115) reagent or monoclonal anti-human CD34 directly conjugated to phycoerythrin (R&D Systems FAB7227P LOT: ACOG01) according to manufacturer protocol.

Techniques: Flow Cytometry, Isolation

Journal: Cells

Article Title: IMG-A1: A Novel Immortalized Granulosa Cell Line for Investigating FSH-Dependent Folliculogenesis and Ovarian Pathophysiology

doi: 10.3390/cells14241940

Figure Lengend Snippet: Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Article Snippet: PE CD34 Rat mAb [RAM34] , Elabscience Biotechnology Co., Ltd., Wuhan, China , E-AB-F1284D.

Techniques: Cell Culture, Staining, Activity Assay, Marker, Immunofluorescence, Flow Cytometry, Control

Journal: International Journal of Molecular Sciences

Article Title: Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics

doi: 10.3390/ijms27010303

Figure Lengend Snippet: Timeline and intermediate characterization of human-induced pluripotent stem cell (iPSC) differentiation into dendritic cells. ( a ) Timeline of human iPSC differentiation into dendritic cells. ( b ) Representative images of cellular morphology during differentiation at days 0, 12, 26, 31, and 32. Human iPSCs were cut into small pieces before seeding (Scale bar represents 200 μm). ( c ) Characterization of human iPSC-derived hematopoietic stem cells by flow cytometry. CD34 and CD45 were used as surface markers. ( d ) Characterization of human iPSC-derived monocytes by flow cytometry. CD14 was used as the surface marker.

Article Snippet: The antibodies used included: CD34-PE (Proteintech, Rosemont, IL, USA, #PE-65183), CD45-PE (BD Biosciences, Ashland, OR, USA, #555483), CD14-FITC (BD Biosciences, Ashland, OR, USA, #555397), CD11c-FITC (Proteintech, Rosemont, IL, USA, #FITC-65086), and CD83-PE (BD Biosciences, Ashland, OR, USA, #550634).

Techniques: Derivative Assay, Flow Cytometry, Marker

Journal: European journal of medical research

Article Title: Olfactory mucosa-mesenchymal stem cells with overexpressed Nrf2 modulate angiogenesis and exert anti-inflammation effect in an in vitro traumatic brain injury model.

doi: 10.1186/s40001-025-02344-6

Figure Lengend Snippet: Fig. 1 Effects of OM-MSCsNrf2 on the levels of relevant cytokines in PC12 cells. A Flow cytometry was used to characterize CD45, CD34, CD29, CD90, CD73, and CD105 of OM-MSCs. B RT-qPCR-based to assess transfection efficiency after overexpression of Nrf2 plasmid. OM-MSCsNrf2: NRF2 overexpression group. OM-MSCs (ctrl): Empty vector transfection group. OM-MSCs: Untransfected OM-MSCs. C Morphology of both OM-MSCs (ctrl) and OM-MSCsNrf2 under the optical microscope. Scale bar: 100 μm. D The changes in cytokine concentrations in PC12 cells measured by ELISA, including IL4, IL10, IL13, VEGF-A, and TGF-β. Results of independent triplicates were expressed as mean ± standard deviation and the statistical difference between two groups was marked with asterisks (“ns” indicates no significant difference, *p < 0.05, and **p < 0.01). OM-MSCs olfactory mucosa-mesenchymal stem cells, Nrf2: Nuclear Factor Erythroid-Derived 2-Like 2, IL interleukin, VEGF-A vascular endothelial growth factor-A, TGF-β transforming growth factor beta

Article Snippet: Identification of OM‐MSCs The isolated OM-MSCs were stained with the following antibodies: phycoerythrin (PE)-labeled anti-CD45 antibody (#202207, 1:500, BioLegend, Inc., San Diego, CA, USA), PE/Cy5.5-labeled anti-CD34 antibody (#NBP233076PECY55, 1:500, Novus Biologicals, Centennial, CO, USA), fluorescein isothiocyanate (FITC)-labeled antiCD29 antibody (#sc-9970 FITC, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), Alexa Fluor® 647-labeled anti-CD90 antibody (#ab317534, 1:500, Abcam, Cambridge, UK), Alexa Fluor® 488-labeled anti-CD73 antibody (#NBP2-71214AF488, 1:500, Novus Biologicals, USA), and allophycocyanin (APC)-labeled anti-CD105 antibody (#NB500-452APC, 1:500, Novus Biologicals, USA) [32].

Techniques: Flow Cytometry, Quantitative RT-PCR, Transfection, Over Expression, Plasmid Preparation, Microscopy, Enzyme-linked Immunosorbent Assay, Standard Deviation, Derivative Assay